In Northern India, nearly 60% of viral hepatitis in pregnant women is attributed to hepatitis E infection (HEV). Given the growing rates of HEV in South Asia and the hypothesis that levels of hormones may affect disease severity in pregnant women with HEV, this study proposes a cross-sectional approach to examine estriol levels and seroprevalence of anti-HEV (IgG and IgM) in village dwellers in Uttar Pradesh, India.